

In order for the tool to run properly, all parts of the base command that are separated by a space need to be entered in separate fields within the Base command section in Tool Editor.

Together, the command and subcommand form the base command. To keep the example simple, we will use the default values for most parameters and options, and aim to build a command line that looks like: samtools sort takes an input BAM-format file containing short DNA sequence reads and sorts it. In this tutorial, we’ll wrap samtools sort, one of the tools in the samtools suite. Step 1: Identify the required options for the command line

#Bam file format example plus
20 minutes plus Platform execution time (approximately 5 minutes with smaller files and 75 minutes with larger files).
